Computational Evaluation of Antibody Binding Efficiency and Conjugation Orientation in Gold Nanoparticles

הרקע לפרויקט:

This project focuses on assessing the efficiency of antibody binding and the orientation of antibody conjugation in gold nanoparticles. By combining computational protein folding analysis with experimental laboratory work, the project aims to evaluate the performance of these nanoparticles under various conditions and their biological effectiveness. The study will leverage advanced statistical methods to compare antibody binding in active and inactive regions and test the functionality of conjugated nanoparticles on cells. This integrative approach will provide valuable insights into optimizing gold nanoparticle-based technologies.

מטרת הפרויקט:

This project aims to enhance the understanding of how antibody conjugation orientation affects binding efficiency. By optimizing the conjugation process and evaluating the functionality of gold nanoparticles, the project aims to improve the effectiveness of these nanoparticles in targeted therapies.

תכולת הפרויקט:

1.  Protein Folding and Binding Analysis:

• Use computational tools (e.g., AlphaFold) to analyze protein folding and assess antibody binding in active versus inactive regions.
• Perform statistical calculations to compare binding efficiencies.

2. Gold Nanoparticle Conjugation:

• Implement techniques for conjugating antibodies to gold nanoparticles.
• Assess and optimize the orientation of antibody conjugation on the nanoparticles.

3. Experimental Testing:

• Design and conduct laboratory experiments to test the functionality of antibody-conjugated gold nanoparticles under various conditions (e.g., temperature, pH).
• Measure the biological efficacy and functionality of the nanoparticles in a cellular environment.

קורסי קדם:

מבוא לביולוגיה למהנדסים

מקורות:

AlphaFold - https://alphafold.ebi.ac.uk
gold nano particles - https://scholar.google.co.il/scholar?hl=iw&as_sdt=0%2C5&q=rachela+popov…