מערכת למדידת טמפרטורת פירוק של DNA
A System for Measuring the Melting Temperature of DNA
The goal of this project is to build a system that can measure the melting temperature of double stranded DNA.
The system will be incorporated into the Advanced Bio-engineering Laboratory.
This project consists of the following parts:
- Building and verifying the electronics, first on a solder less breadboard, and then on a printed circuit prototyping board.
- Once the electronics are verified, we would like to then design a custom printed circuit board (using tools such a KiCAD or PCB Express), and have such a board manufactured.
- Improve the LabView control program for this system. This includes adding an auto zero function, converting the control loop to a finite state machine implementation, and implementing the system under Linux.
- Using the system to measure DNA melting behavior. The system will be test on DNA solutions of 80bp, 40bp, and 20bp fully matched strands, 20bp 50% mismatched strands, and 20 bp fully mismatched (i.e. different random) strands.
Labview knowledge, and a desire to work with analog electronics.
The documentation for the first generation of this system can be found on the 83411 moodle site.