Multimodal Portable Optical Neural Imaging
Optical techniques are widely used in clinical settings and in biomedical research to interrogate bio-molecular interactions and to evaluate tissue dynamics. Miniature integrated optical systems for sensing and imaging can be portable, enabling long-term studies in living tissues.
We present the development of a compact multi-modality optical neural imaging system, to image tissue blood flow velocity and oxygenation, using a fast CCD camera and miniature VCSEL illumination. We combined two techniques of laser speckle contrast imaging (LSCI) and intrinsic optical signal imaging (IOSI) simultaneously, using these compact laser sources, to monitor induced cortical ischemia in a full field format with high temporal acquisition rates. We have demonstrated tracking seizure activity, evaluating blood-brain barrier breaching, and integrating fast spatial light modulators for extended imaging depth and auto-focusing during brain imaging of flow dynamics. Finally, we will present our prototype designs and system optimization and evaluation for a low-cost portable imaging system as a minimally invasive method for long-term neurological studies in un-anesthetized animals. This system will provide a better understanding of the progression and treatment efficacy of various neurological disorders, in freely behaving animals.