Development of an Advanced Fluorescence Assay for Precise and Reliable Quantification of Antibodies on Particles

הרקע לפרויקט:

This project aims to develop and refine a method for quantifying the amount of antibodies conjugated to particles using fluorescence techniques. By employing advanced fluorescence assays, the project seeks to provide a precise and reliable quantification approach for antibody-particle conjugates. This method will be instrumental in various applications, including biomedical research and diagnostics, where accurate measurement of antibody levels on particles is crucial for assessing their functionality and efficacy.

מטרת הפרויקט:

The purpose of this project is to establish a fluorescence-based method that accurately quantifies the concentration of antibodies attached to particles. This will enhance the ability to monitor and control the quality of antibody-particle conjugates, leading to more effective applications in research, diagnostics, and therapeutic developments.

תכולת הפרויקט:

1. Fluorescence Method Development:
- Research and select appropriate fluorescence techniques and dyes for antibody quantification.
- Develop and optimize fluorescence assays to measure antibody concentration on particles.
- Validate the method's sensitivity, specificity, and accuracy.
2. Experimental Setup:
- Prepare and characterize particle-antibody conjugates.
- Design and implement experiments to test the fluorescence-based quantification method.
- Use control samples and standards to calibrate and validate the assay.
3. Data Analysis:
- Analyze fluorescence data to determine the concentration of antibodies on particles.
- Perform statistical analysis to ensure the accuracy and reliability of the results.
- Compare the fluorescence-based method with traditional quantification techniques.

קורסי קדם:

מבוא לביולוגיה למהנדסים, דימות רפואי

מקורות:

מאמרים על ננו חלקיקי זהב וצימוד לנוגדנים: https://scholar.google.co.il/scholar?hl=iw&as_sdt=0%2C5&q=rachela+popov…