Development of a Novel Biolistic Approach for Drug Delivery
Abstract: Biolistic delivery is a promising route for delivering payloads into both cells and tissue. A novel biolistic device based on a pneumatic capillary gene gun was developed for fast, accurate and highly localized delivery. The technique enables delivery into biological samples in vitro and in vivo without damage to the targeted area. Currently this method is used for gene manipulation by delivering particles coated with DNA or RNA molecules. Recently, a second generation gene gun has been introduced enabling higher accelerating Helium pressures. Conventionally this set up has been used to deliver cargos conjugated to heavy metal carriers. In the present study, we developed the ability to launch non-metal highly porous micro-particles and nano sized carriers that are valuable drug carriers. We characterized the gene gun performance for a variety of carriers and a controlled delivery methodology was developed. By optimizing the gun parameters e.g., the accelerating gas pressure, distance from preparation and dose, we have successfully delivered nano sized Iron-oxide particles and porous Silicon carriers. The latter reached deep targets and crossed a skin barrier in a highly spatial resolution. Moreover, in this study, a new paradigm for an effective delivery of therapeutics into cancer cells is presented. In our study, degradable porous silicon carriers, which were tailored to carry and release a model anti-cancer drug, were biolistically bombarded into in-vitro cancerous targets. The improved gene gun model enables an efficient delivery of smaller or lighter particles to penetrate deeper into a tissue than was previously possible. The unique combination of biolistics with the temporal control of payload release from porous carriers presents a powerful and non-conventional platform for designing new therapeutic strategies and may open new possibilities for in- vivo delivery trials.